Khulna University (Bengali: খুলনা বিশ্ববিদ্যালয়) is a public university in Bangladesh. It is situated at Gollamari, Khulna, Bangladesh, by the river Moyur, beside the Khulna-Satkhira highway. The academic programs of Khulna University started on 31 August 1991 with 80 students in four disciplines. As of November 2019, the university has 29 disciplines under six schools and two institutes. It is the only public university in Bangladesh where student politics is not allowed.
In 1974, the Kudrat-e-Khuda Commission stated the importance of establishing a public university in Khulna Division in its final report. The government finalized the programme of establishing a university at Khulna on 4 January 1987 and the Khulna University Act was passed in the Bangladesh National Parliament in July 1990. Distinctive law named খুলনা বিশ্ববিদ্যালয় আইন (The Khulna University Act, 1990) passed in the parliament empowers the university. The university started its formal inauguration of academic activities on 25 November 1991.